Discovery proteomics is a good starting point to evaluate the feasability of a project and to further design an analytical strategy.

It is the method of choice for proteome mapping and identification of PTMs.

We can deploy tailor-made analytical methods that meet the needs of your project. Relative quantification between samples is possible.

Applications that require discovery analysis

• Protein sequence identification
• Proteome mapping
• Label free quantification
• Interactome studies
• Identification of post-translational modifications

 

 

 

Fast 

 

Standard

 

Deep

 

Multidimensional

Throughput per sample

 

Fast-UHPLC (10 to 30min)

 

Standard gradient (90min)

 

Long gradient (240 to 300 min)

 

Up to 50 h

GMass spectrometer

 

Q Exactive HF

 

Q Exactive plus 
LTQ orbitrap Velos

 

Q Exactive plus
LTQ orbitrap Velos

 

Q Exactive plus
LTQ orbitrap Velos

Indicative number of protein IDs*

 

700-1500

 

2500

 

5000

 

9000

Applications

 

- High throughput screening of large sample cohort
- Low to medium complexity samples

 

- Time and cost effective approach

 

- Comprehensive study of a limited number of samples/conditions

 

- Tailored options on demand
- Extensive proteome mapping 

*based on hela cell lysate